Recombinant Human IL-2 Protein (202-IL)

Recombinant Human IL-2 Protein Summary

Purity >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level <0.10 EU per 1 μg of the protein by the LAL method.
Activity Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED50 for this effect is 0.05-0.25 ng/mL.
The specific activity of Recombinant Human IL-2 is approximately 2.1 x 104 IU/μg, which is calibrated against recombinant human IL-2 WHO International Standard (NIBSC code: 86/500).
Source E. coli-derived human IL-2 protein
Ala21-Thr153, with an N-terminal Met
Accession # P60568

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